mgu.ac.in B.Sc Biotechnology Question Paper Model : Mahatma Gandhi University

Name of the University : Mahatma Gandhi University
Degree : B.Sc. Fifth Semester
Name Of The Subject : Biotechnology
Document type : Model Question Paper

Website : mgu.ac.in
Download Model Question Papers : https://www.pdfquestion.in/uploads/7519-biotechnology.docx

Biotechnology Model Paper :

Fifth Semester : For B. Sc. Biotechnology
I.Answer all questions :
1. What is RNase H?
2. What is c DNA?

Related : Mahatma Gandhi University Chemical Engineering–II B.Tech Model Question Paper : www.pdfquestion.in/5373.html

3. Define insertional inactivation
4. What is a shuttle vector?
5. Write down the function of polynucleotide kinase.
6. What is microinjection?
7. What is plasmid incompatibility?
8. Write down the restriction sequence of Eco R1 and Bam HI
9. What are isoschizomers?
10. Write the principle behind CaCl2 mediated gene transfer

II.Answer any eight of the following :
11. What is chromosome walking?
12. What is a Binary vector?
13. Briefly explain the features of CaMV vectors.
14. What are biolistics?
15. Differentiate between marker genes and reporter genes.
16. What is dot blotting?
17. What is alpha complementation?
18. Write the advantages of M13 vectors
19. What are type I restriction enzymes?
20. What is the significance of Klenow fragment?
21. What is reverse transcriptase?
22. What is a cosmid?

III. Answer any six of the following :
23. What is terminator gene technology? Explain the mechanism involved in the control of plant gene expression.
24. Briefly explain concept of molecular pharming.
25. Describe the various stages involved in performing PCR experiment.
26. Write a note on human genome project.
27. What are the different stages in the construction of genomic library?
28. Discuss the various desirable properties of an ideal vector.
29. Discuss the various steps in the production of recombinant insulin.
30. Compare pBR32 and YAC vector systems
31. Discuss the different types of PCR techniques used in Molecular Biology

IV. Answer any two of the following : ( 15 marks each)
32. Explain the various steps involved in the creation of transgenic plant using Agrobacterium. Discuss the features of various plant vectors used in plant Recombinant DNA experiments.
33. What are the various applications of Recombinant DNA technology? Discuss in detail how this technique is useful in medical applications?
34. Discuss the various methods of gene transfer in plants. Specify the merits and demerits of each method.
35. Write an essay on plasmid vectors.

Industrial Biotechnology :
I.Answer all questions :
1. What are auxotrophs?
2. What is crowded plate technique?
3. What is freeze drying?
4. What is an air lift fermentor?
5. What is tachometer?
6. What are baffles?
7. Specify the advantages of bioprocess over chemical process ?
8. What is market potential of a product?
9. Name the industrially important microbe used in the production of vitamin B12.
10. Specify the application of check valves used in bioreactor designing. (1×10 = 10 marks)

II. Answer any eight of the following :
11. What is sonication? Discuss its application in bioprocess?
12. What is secondary screening? Name some of the secondary screening methods used in bioprocess.
13. Discuss the importance of drying in bioprocess. Name some of the drying methods used for fermentation products.
14. Give the schematic representation of a CSTR?
15. What are spargers? Specify the different types of spargers used in bioreactors.
16. Explain the advantages and disadvantages of defined and undefined media used in bioprocess.
17. What is a continuous culture? Discuss the applications of continuous culture in bioprocess.
18. What is specific growth rate ? Specify how it can be estimated in a batch process.
19. What are chelators used in fermentation media. Give an example.
20. What are antiform agents used in fermentation media? Give an example.
21. What is dialysis? Explain its applications in industrial Biotechnology.
22.What is gelfiltration? How is it useful in the purification of proteins?

III. Answer any six of the following :
23. Discuss the various stages in the fermentation production of aminoacids.
24. Discuss the significance of centrifugation technique in bioprocess.
25. Discuss the various methods of cell disruption. Explain the principle behind each method.
26. Explain the various advantages of bioprocess. Specify some of the significant applications of bioprocess.
27. Explain various types of online and offline instruments connected to bioreactor. Comment on their applications.
28. What is a packed bed reactor? Comment on its application.
29. What is solid state fermentation? Specify its applications.
30. What is cell immobilisation? Comment on its applications in bioprocess.
31. What are inducers and inhibitors used in media designing in fermentation? (4×6 = 24 marks)

IV. Answer any two of the following :
32. Discuss the various methods of strain improvement. Explain each method with suitable examples.
33. What are the various methods of downstream processing? Discuss the principle behind each method.
34. Explain the significant functions of various components in a typical bioreactor. Discuss the various types of bioreactors used in fermentation process.
35. Discuss the various stages in the fermentation production of antibiotics