BT602 Bioseparation Technology B.Tech Question Paper : wbut.ac.in
Name of the University : West Bengal University of Technology
Department : Bio-Technology
Degree : B.Tech
Sem : VI
Subject Code/Name : BT-602/Bioseparation Technology
Website : wbut.ac.in
Document Type : Previous Year Examination Question Paper
Download Model/Sample Question Paper :
2009 : https://www.pdfquestion.in/uploads/qpaper.wbut.ac.in/6739-2009BT-602.pdf
2010 : https://www.pdfquestion.in/uploads/qpaper.wbut.ac.in/6739-2010BT-602(1).pdf
2011 : https://www.pdfquestion.in/uploads/qpaper.wbut.ac.in/6739-2011BT-602(2).pdf
2012 : https://www.pdfquestion.in/uploads/qpaper.wbut.ac.in/6739-2012BT-602-(6118).pdf
WBUT Bioseparation Technology Question Paper
Engineering & Management Examinations, June – 2009 :
Semester – 6 :
Time : 3 Hours
Full Marks : 70
Related : West Bengal University of Technology Immunology B.Tech Question Paper : www.pdfquestion.in/6737.html
GROUP – A
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the following : 10 × 1 = 10
i) In gel filtration chromatographic separation, bio-molecules are separated based on what property of bio-molecules ?
a) Size b) Charge
c) Hydrophobic interaction d) Metal ion affinity.
ii) To purify Lac-repressor from E. c o l i cell extract by affinity column chromatography, what type of ligand molecule will be immobilized to the gel matrix ?
a) Lac promoter RNA b) Lac operator DNA
c) Lac Z mRNA d) Lac I mRNA.
iii) By Ni-NTA-Agarose affinity column chromatography what type of protein can you purify ?
a) GST-tagged protein b) 6 X His-tagged protein
c) Cys-tagged protein d) DNA binding protein.
iv) Molecular weight of a protein can be determined by
a) Size exclusion chromatography
b) Ion-exchange chromatography
c) Pseudo-affinity chromatography
d) Affinity chromatography.
v) Cell disruption in homogenizer is based on
a) applied voltage b) operating pressure
c) salt concentration d) osmosis.
vi) Pervaporation involves change of
a) mass volume b) isoelectric pH
c) applied voltage d) Phase.
vii) Basic principle of centrifugation depends on
a) concentration b) polarization
c) centripetal force d) pressure gradient.
viii) In affinity chromatography, if the reactive group on the matrix is – OH group, then coupling agent is
a) Bisepoxide b) Dichlorotriazine
c) Tricyclic chloride d) Cyanogen bromide.
ix) Ultrafiltration is used for separation with molecular weight range from
a) 0.1 – 10 µm b) 10 – 100 µm
c) 100 – 200 µm d) 200 – 500 µm.
x) Which method is commonly used to separate inhibitory fermentation product such as ethanol from fermentation broth ?
a) Aqueous two phase extraction b) Liquid-Liquid extraction
c) Adsorption d) Ultrafiltration.
xi) In reverse osmosis, the deposition of solute molecules on membrane surface results in large resistance for solvent flow. This phenomenon is known as
a) Reflection coefficient b) Rejection coefficient
c) Break through point d) Concentration polarization.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following. 3 × 5 = 15
2. Discuss the downstream processing steps in the production of intracellular enzyme from fermentation broth. 5
3. Write a short note on Pseudo-affinity chromatography. 5
4. Discuss the theoretical principles and practice of salting out of proteins by ammonium sulphate. 5
5. A centrifuge having a radius of bowl of 100 mm is rotating at 1000 r.p.m. Calculate the centrifugal force developed. 5
6. A mixture of two proteins A and B have to be separated by gel chromatography. The partition coefficient ( KD ) for A is 0.5 and for B is 0.15. The void volume ( Vo ) in the column is 20 cm 3 and the included volume ( Vi ) within the gel particle is 30 cm 3 . The total volume of the column is 60 cm 3 . The flow rate of eluent is 100 cm 3 /h. Ignoring dispersion and other effects, how long will it take for A to exit the column ? How long is for B ? 5
GROUP – C
( Long Answer Type Questions )
Answer any three questions. 3 × 15 = 45
7. Write short notes on any three of the following : 3 × 5
a) Affinity chromatography
b) Non-mechanical methods of cell disruption
c) Hyper-filtration
d) Pervaporation.
8. a) What do you mean by membrane separation ? Name the different types of membrane separation processes. 1 + 2
b) Write short notes on any four of the following : 4 × 3
i) Reverse osmosis.
ii) Ultra-filtration.
iii) Membrane fouling.
iv) Concentration polarization.
v) Hollow fibre module.
9. a) Describe different types of Chromatographic separation technique available for the separation of metabolic products present in fermentation broth. 10
b) What are the basic principles exploited for the separation of biomolecules by chromatographic process ? 5
10. a) Aqueous two-phase extraction is used to recover alpha-amylase from solution. A polyethylene glycol-dextran mixture is added and the solution separates into two phases. The partition coefficient is 4.2. Calculate the maximum possible enzyme recovery when
i) the volume ratio of upper to lower phases is 5.0 and
ii) the volume ratio of upper to lower phases is 0.5. 7
b) Cell free fermentation liquor contains 8 × 10 – 5 mol / lit. immunoglobulin G. It is proposed to recover at least 90% of this antibody by adsorption on synthetic, non-polar resin. Experimental equilibrium data are correlated as follows
AS is mol solute adsorbed per cm 3 adsorbent and C*
A is liquid phase conc. in mol/lit. What minimum quantity of resin is required to treat 2 m 3 fermentation liquor in a single-stage mixed tank ? 8
11. Describe in brief the operations involved for the isolation and purification of ethanol or any organic acid from commercial plant. ( Complete flowchart is a must ) 15