BT403 Molecular Biology & R-Dna Technology B.Tech Question Paper : wbut.ac.in
Name of the University : West Bengal University of Technology
Department : Bio-Technology
Degree : B.Tech
Sem : IV
Subject Code/Name : BT-403/Molecular Biology And R-Dna Technology
Website : wbut.ac.in
Document Type : Previous Year Examination Question Paper
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2009 : https://www.pdfquestion.in/uploads/dspace.jdvu.ac.in/6728-2009BT-403.pdf
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Molecular Biology And R-Dna Technology Question Paper :
Engineering & Management Examinations, June – 2009 :
Semester – 4 :
Time : 3 Hours
Full Marks : 70
Related : West Bengal University of Technology BT402 Industrial Microbiology & Enzyme Technology B.Tech Question Paper : www.pdfquestion.in/6727.html
GROUP – A :
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the following : 10 × 1 = 10
i) Polycistronic mRNA is found in
a) prokaryotes b) plants
c) lower eukaryotes d) all living cells.
ii) The factor(s) responsible for initiation and termination of transcription in E.coli is/are
a) s and ? respectively b) ? and s respectively
c) s only d) ? only.
iii) Repressor molecule binds to the site of DNA called
a) Operator b) TATA box
c) Pribnow box d) CAP binding site.
iv) Restriction enzyme which cuts the double stranded DNA inside its recognition site is
a) Type-I b) Type-II
c) Type-III d) Type-IV.
v) Enzyme used in 5’ end labelling of DNA is
a) Alkaline phosphatase b) Polynucleotide kinase
c) DNA polymerase d) Terminal transferase.
vi) Of the sequences shown below, which would be the most likely target sequence for a restriction endonuclease ?
a) TATAAT b) CATTAC
c) GCCTGC d) GAUUAC.
vii) Which plasmid of the following would be the best cloning vector ?
a) pHA 1 : 12 restriction sites for EcoR1 and 2 for PstI;
viii) Which type of vector can be transformed into both bacterial and yeast cells ?
a) Cosmid b) YAC
c) Shuttle vector d) Expression vector.
ix) In a cell with alacOc mutation, the operon genes will
a) only be expressed in the presence of lactose
b) not be expressed in the presence of lactose
c) not be expressed in the absence of lactose
d) be expressed in the presence or absence of lactose.
x) An enhancer element is
a) a specific upstream nucleotide sequence of a gene
b) a regulatory protein
c) an mRNA degrading enzyme
d) an mRNA transcript.
xi) The transcriptional start site and translational start site are
a) same b) different
c) not specified d) none of these.
xii) If the template strand of a segment of a gene has the nucleotide sequence 5’CGAATCG3’, the sequence present in the RNA transcript will be
a) 5’CGAUUCG3’ b) 3’GCUUAGC5’
c) 5’CGAAUCG3’ d) 3’GCUAAGC5’.
GROUP – B : ( Short Answer Type Questions )
Answer any three of the following questions. 3 × 5 = 15
2. Write the r eaction and only one use of each of the following enzymes in r-DNAtechnology :
i) Klenow fragment
ii) Bacterial alkaline phosphatase
iii) Terminal deoxynucleotide kinase
iv) T4 DNA ligase
v) Poly nucleotide kinase.
3. Write short note on any one of the following :
i) 5’ Capping of eukaryotic mRNA
ii) mRNA splicing
iii) 3’ poly adenylation.
4. Write short note on any one of the following :
i) pBR322
ii) pUC18
iii) Cosmid.
5. a) The restriction endonuclease EcoRI recognizes the sequence GAATTC. If a 40·96 kb genomic DNA with random sequence digested with EcoRI, theoretically how many fragments will be produced ? ( presume that 50% GC content in the genomic DNA )
b) What are HRE ? Where are the receptors for steroid hormones and non-steroidhormones found in a cell ? 2
6. Write a short note on Wobble hypothesis. State how occurrence of at least three tRNAs can account for six codons for Serine. 4 + 1
GROUP – C :
( Long Answer Type Questions )
Answer any three of the following questions. 3 × 15 = 45
7. a) What is cDNA library ? What is the difference between cDNA library and genomic library ?
b) If you know the protein product of a eukaryotic gene, then schematically draw a method for cloning and selection of the full length cDNA molecules of the corresponding gene.
c) A vector is used to clone 20 kb DNA fragments from human genome let you wish to isolate a gene containing completely on that 20 kb fragment. To have a 99% chance of isolating the gene from recombinant genomic library, how many independent clone must be examined ? 3 + 7 + 5
8. a) What is operon ?
b) Explain with diagram the mode of operation of lac operon
i) in absence of lactose,
ii) in presence of lactose.
c) Explain how the regulatory protein Ara C acts as a repressor and activators of arabinose operon.
d) Write one application of lac operon in r-DNA technology. 1 + 8 + 4 + 2