BT302 Biochemistry B.Tech Question Paper : wbut.ac.in
Name of the University : West Bengal University of Technology
Department : Bio-Technology
Degree : B.Tech
Sem : III
Subject Code/Name : BT-302/Biochemistry
Website : wbut.ac.in
Document Type : Previous Year Examination Question Paper
Download Model/Sample Question Paper :
2011 : https://www.pdfquestion.in/uploads/dspace.jdvu.ac.in/6725-2011BT%20-%20302(2).pdf
2012 : https://www.pdfquestion.in/uploads/dspace.jdvu.ac.in/6725-2012BT-302..pdf
Biochemistry Question Paper :
Time Allotted : 3 Hours
Full Marks : 70
** The figures in the margin indicate full marks.
** Candidates are required to give their answers in their own words as far as practicable.
Related : West Bengal University of Technology BT303 Structural Chemistry of Biomolecules B.Tech Question Paper : www.pdfquestion.in/6723.html
GROUP – A :
( Multiple Choice Type Questions )
1. Choose the correct alternatives for the following : 10 × 1 = 10
i) Which of the following is Not a typical event associated with cell signalling ?
a) Activation of G-proteins by exchanging GTP for GDP
b) Production of the second messengers cAMP and IP3
c) Stimulation of apoptosis
d) Activation of protein kinases.
ii) Estrogen and testosterone are steroid hormones, and are most likely to bind to
a) membrane ion channel
b) enzyme linked membrane receptor
c) G-protein linked membrane receptor
d) cytoplasmic receptor.
iii) Acetyl CoA is produced by
a) Pentose phosphate pathway
b) Beta oxidation
c) TCA cycle
d) None of these.
iv) In plants, under anaerobic conditions pyruvate is converted into
a) Ethanol b) Lactate
c) Water d) Acetyl CoA.
v) ß-alanine is the degraded product of
a) thymidine b) cytidine
c) aspartic acid d) cholesterol.
vi) Three amino acids that donate amino groups for the purine biosynthesis are
a) glycine, glutamine, aspartate
b) glycine, beta alanine, aspartate
c) glycine, alanine, aspartate
d) lysine, glutamine, aspartate
e) lysine, glutamate, asparagine
f) lysine, glycine, asparagine.
vii) Inactive precursors of some enzymes that are activated through hydrolysis reactions are called
a) allosteric enzymes
b) apoenzymes
c) holoenzymes
d) prosthetic groups
e) zymogens.
viii) TCA cycle is
a) catabolic b) amphibolic
c) anabolic d) cyclic.
ix) Example of a second messenger is
a) cAMP
b) ATP
c) GTP.
x) Nitric oxide and urea have in common the fact that they both have as an immediate precursor amino acid
a) aspartate b) arginine
c) glutamate d) phenyl alanine.
GROUP – B : ( Short Answer Type Questions )
Answer any three of the following. 3 × 5 = 15
2. Describe in brief the pentose phosphate pathway.
3. Briefly explain the effect of a bacterial toxin on G-protein.
4. Write down a short note on allosteric regulation.
5. What are the end products of odd carbon fatty acids after complete ß-oxidation ? Explain with reactions.
6. How glycogen breakdown has been stimulated in response to hormone action ?
GROUP – C : ( Long Answer Type Questions )
Answer any three of the following. 3 × 15 = 45
7. Classify enzymes in six different categories with examples. What do you mean by enzyme unit and specific activity ? Differentiate coenzyme and prosthetic group. Explain the effect of pH and temperature on enzyme activity. 6 + 3 + 2 + 4
8. Explain the main three extracellular signalling types with examples. Define second messenger with example. Explain the role of a second messenger regarding glycogen metabolism. What do you mean by cellular adhesion ? 6 + 1 + 6 + 2
9. In a diagrammatic representation, describe all steps of TCA cycle with the structure of the intermediates mentioning the enzymes and the cofactors. Briefly describe in a flow chart how acetyl CoA is produced from pyruvate ? 7 + 3 + 2 + 3
10. Mention catabolic pathway of phenyl alanine. What defect in this pathway results in phenyl ketonuria ? Discuss urea cycle. Discuss how C4 plants share advantage over C3 plants. 4 + 1 + 5 + 5
11. Discuss about any one disorder of amino acid metabolism. Write down a short note on transamination. Describe catabolism of tyrosine. “Protein turnover is tightly regulated.” Explain. 4 + 4 + 4 + 3
Biochemistry – 2012 :
Time Allotted : 3 Hours
Full Marks : 70
** The figures in the margin indicate full marks.
** Candidates are required to give their answers in their own words as far as practicable.
GROUP – A : ( Multiple Choice Type Questions )
1. Choose the correct alternatives for the following : 10 × 1 = 10
i) The net gain of ATP molecules resulting from Glycolysis is
a) 2 b) 4
c) 36 d) 38.
ii) TCA cycle takes place at
a) nucleous b) mitochondria
c) cytosol d) chloroplast.
iii) The storage form of carbohydrate in animal is
a) starch b) cellulose
c) glycogen d) glucose.
iv) The largest energy reserve (kilocalories) in humans is
a) blood glucose
b) liver glycogen
c) muscle glycogen
d) adipose tissue triglyceride.
v) The coenzyme involved in transfer of carboxyl group
a) NADH
b) coenzyme A
c) S-adenosyl methionine
d) biotin.
vi) Acyl Carrier Protein takes part in
a) amino acid degradation
b) glycolysis
c) fatty acid biosynthesis
d) fatty acid degradation.
vii) The molecule which does not contain a high-energy bond is
a) ATP b) AMP
c) ADP d) ppi.
viii) Glycolytic pathway regulation involves
a) allosteric stimulation by ADP
b) allosteric inhibition by ATP
c) feedback, or product, inhibition by ATP
d) all of these.
ix) Glycogen has
a) a -1, 4 linkage
b) a -1, 6 linkages
c) a -1, 4 and a -1, 6 linkages
d) a -1, 4 and ß -1, 6 linkage.
x) The lipid bilayer is impermeable to
a) hydrocarbons
b) hydrophobic molecules
c) small uncharged polar molecules
d) large uncharged polar molecules.
GROUP – B : ( Short Answer Type Questions )
Answer any three of the following : 3 × 5 = 15
2. How Vibrio cholerae toxin reacts with our body ?
3. Describe in brief the process of omega oxidation.
4. What is glyoxylate cycle ? Name two key enzymes of glyoxylate cycle and write the reactions catalyzed by these enzymes. 1 + 4
5. What do you mean by transamination ? Discuss the role of vitamin B6 in transamination. 2 + 3
6. Discuss the induced fit hypothesis to describe the enzymeaction.