Name of the Board : CBSE Academic
Class : XII STD
Subject : Biotechnology
Year : 2015 – 16
Website : http://cbseacademic.in/SQP_CLASSXII_2015_16.html
Download Sample Question Paper : https://www.pdfquestion.in/uploads/8024BioTech.pdf
Download Marking Scheme : https://www.pdfquestion.in/uploads/8024BioTevhMS.pdf
Biotechnology Sample Question Paper :
Time: 3 Hrs.
M.M.:70
Related : CBSE Academic Class XII Biology Sample Question Paper 2015-16 : www.pdfquestion.in/8010.html
General Instructions :
(i) All questions are compulsory.
(ii) There is no overall choice. However, an internal choice has been provided in one question of two marks, one question of three marks and all three questions of five marks. You have to attempt only one of the choices in such questions.
(iii) Question paper contains four sections -A, B, C and D.
(iv) Question numbers 1 to 6 are very short answer questions, carrying 1 mark each.
(v) Question numbers 7 to 14 are short answer questions – I, carrying 2 marks each.
(vi) Question numbers 15 to 25 are also short answer questions – II, carrying 3 marks each.
(vii) Question numbers 26 to 28 are long answer questions, carrying 5 marks each.
(viii) Use of calculators is not permitted. However, you may use log tables, if necessary.
Section-A :
Q1. A scientist wants to protect a part of a DNA from attack by restriction enzymes. Design first step of his experiment? (1)
Q2. Presence of selectable marker is an essential feature of an ideal cloning vehicle. Give reason. (1)
Q3. Expand and define PER. (1)
Q4. Given below is a list of the first 8 residues of the ß-helix in myoglobin from different organisms. Based on this information, identify the amino acids which are most
(a) conserved (b) variable
Q5. In assessing the effect of growth factors on animal cell cultures, a particular phase of growth is more suitable. Name this phase giving reason for the same. (1)
Q6. CHO animal cell line is used to express r-HuEPO. Give reason. (1)
Section-B :
Q7. pUC19 and a linear DNA (both of same size) have two sites for the same restriction – endonuclease. When cleaved and separated on agarose gel electrophoresis, how many bands will be visualized in each case and why?
Q8. Chymotrypsin catalyses the hydrolysis of proteins containing bulky, aromatic, hydrophobic amino acids.Comment. (2)
Q9. Interpret possible use of nick translation in detecting chromosomal translocations. Draw a suitable diagram. (½ x4)
Q10. C.elegans is a eukaryotic organism with a genome of 97 Mb and about 20,000 genes. Why organizational features of this genome are unusual when compared to the genomes of other eukaryotes, such as yeast and Drosophila?
OR
Blast helps in finding the homology in biological sequence alignment. Give any two reasons for this homology.
Q11. Highlight graphically the differences between culturing microbes in the school laboratory and a bioreactor which allow cells to grow in a continuous culture system. (1+1)
Q12. State any two advantages of using Pichia pastoris as a eukaryotic expression host. (1+1)
Q13. Execute the engineered biosynthetic pathway which leads to the production of valuable secondary metabolites for their over-production. (2)
Q14. Enlist the advantages and limitations of animal cell culture (two each). (½ x4)
Section-C :
Q15. Parth has identified luciferase gene from firefly. Construct a flowchart of the steps that you would follow to transfer the gene to a plant. (½x6)
Q16. One of the major uses of genome sequencing is to develop tools for future experimentation. Given the sequence of a ribonuclease gene from the model plant
Brassica, how would you design a tool for isolating the ribonuclease gene from an Oryza sativa plant. (½x6)
Q17. Linus Pauling predicted that ScHb differed in charge from normal hemoglobin protein. Explain the technique which was used to confirm this with the help of diagram. (½x6)
Q18. If a given protein with a molecular weight of 20,000 daltons containing 5,4,3,2, and 1 charges, is subjected to mass spectrometry, find the sequence of protein ions detected by the mass spectrometer.
Q19. A patient suffering with blood cancer has been put on radio and chemotherapy for the past 5 months. How can FISH technique be used to monitor the effect of the same? (1+1+1)
Q20. Annotation of human genome sequence reveals that our genome contains 30000- 33000 genes. Proteomic analysis indicates that human cells are capable of synthesizing more than 30,000 different proteins. How can this discrepancy be reconciled?