Name of the University : Ananda Rangapillai Library Pondicherry University
Degree : M.Sc.
Name Of The Subject : Biotechnology
Document type : Entrance Exam Model Paper
Website : http://lib.pondiuni.edu.in/
Download Model Question Paper: https://www.pdfquestion.in/uploads/7554-M.ScBiotechnology.pdf
LIB Biotechnology Model Paper
Which oi the following methods for studying loss of gene function does-not involve any modification of the genome?
a.gene knockout by homologous recombination
b.RNA interference by injection of double stranded DNA
c.expression of an integrated antisense transgene
d.all the above
Related : Ananda Rangapillai Library Pondicherry University M.Sc Biochemistry & Molecular Biology Paper Model : www.pdfquestion.in/7552.html
Restriction fragment length polymorphism (RFLP) is
(A) the technique used to fingerprint of inheritance I
(B) the difference in the restriction maps between two individuals of . one species
(C )the difference in the restriction maps between two individuals of two species ‘ I
(D) the difference in the restriction maps between the two alleles in a diploid cell
A reporter gene
(A)acts as repressor
(B)allows gene expression to be readily measured
(C)enhances mRNA stability I
(D)interacts with RNA polymerase
Transaction has been used extensively for genome mapping of bacteria. which of the following process is useful for gene mapping? ‘
(A)bacterial lysis
(C)specialized transaction
(B)’1 generalized transaction I
(D)site speci?c recombination
Two double stranded DNA samples that are identical with respect to the number of base pairs, but differ signi?cantly in their GC content, can be separated by
(A) dialysis (B)agarose gel electroscopes
(C) density gradient centrifugation (D) oligo-dT column chromatography
Pyrosequencing derives its name from the fact that
(A) the bases are detected by pyrolysis it detects pyrophosphate released during base incorporation
For glycoproteins, most commdnly used probe is
(A) antibody
(B)antigens
(C) interferons
(D) lectin
Phage M13 vectors are widely used for
(A) obtaining fragments of cloned DNA suitable for DNA sequencing
(B) obtaining single stranded copies of cloned DNA suitable for DNA sequencing
(C) obtaining double stranded copies of cloned DNA suitable for electrophoresis
(D) obtaining double stranded copies of cloned DNA suitable for DNA sequencing
Vectors designed to replicate in cells of two different species are called
(A) phasmids
(B) phagemids
(C) transfer vectors
(D) shuttle vectors
The injection of DNA into developing in florescence using a hypodermic syringe is – called ‘ – –
(A) microinjection
(B) microfection
(C) micromanipulator
(D) DNA delivery
A major advantage of monoclonal antibodies compared to monoclonal antibodies is that monoclonal antibodies
(A) have highersingfinity binding to’antigens .
(B) _ have identical binding sites that recognize a specific epitope
(C) cross—link molecules that share antigenic sites i
(D) are more easily coupled with probes such as fiuorescent dyes
Biochemistry And Molecular Biology :
Pernicioiis anemia is developed due to deficiency of
(A) Erythropoetin (B) Vitamin B12
(C) Iron (D) Vitamin BB
Choose the drug which is a HZ-receptor antagonist
(A) Omeprazole (B) V Pirenzepine
(C) Carbenoxolone _ (D) Ranitidine
Vasopressin possesses the following
(A) Antidiuretic property
(B) Vasodilatation property
(C) Release of a thyroid hormone into the plasma
(D) Diuretic property
Loosening of teeth, gingivitis andhemorrhage occur in the de?ciency of
(A) VitaminR (B) VitaminBl
(C) Vitamin B6 (D) Vitamin c
Mechanism of Rifampin action is
(A) Inhibition of mycolic acids synthesis
(B) Inhibition of DNA dependent RNA polymerase
(C) Inhibition of topoisomerase II
(D) Inhibition of camp synthesis
Half life (t 1A) is the time required to
(A) Change the amount of a drug in plasma by half during elimination
(B) Metabolize a half of an introduced into the actiire metabolite
(C) Absorb ahalf of an introduced drug
(D) Bind a half of an introduced to plasma proteins
Fasting plasma true glucose level is
(A) 70-110 rug/100ml (B) 80-120 mg/lOOml
(C) 100-140mg/100m1 (D) 1.50-250mg/100ml
Diabetes mellitus can be detected by which test using urine specimen
(A) Ruthera’s test (B) Benedict’s test ‘
(C) Hay’s test _ (D) Benzidine test
Blood cholesterol is elevated in which of the following condition
(A) Hypothyroidism (B) Hyperthyroidism
(C) Hypoinsulinism (D) None of the above
Estimation of serum triglycerides is of diagnostic importance in
(A) Multiple myeloma (B) Hyperlipidemias
(C) Nephrotic syndrome (D) Jaundice
Fasting plasma true glucose level is
(A) 70-110 rug/100ml (B) 80-120 mg/lOOml
(C) 100-140mg/100m1 (D) 1.50-250mg/100ml
Diabetes mellitus can be detected by which test using urine specimen?
(A) Ruthera’s test (B) Benedict’s test ‘
(C) Hay’s test _ (D) Benzidine test