Name of the University : West Bengal University of Technology
Department : Bio-Technology
Degree : B.Tech
Sem : IV
Subject Code/Name : BT-402/Industrial Microbiology & Enzyme Technology
Website : wbut.ac.in
Document Type : Previous Year Examination Question Paper
Download Model/Sample Question Paper :
2009 : https://www.pdfquestion.in/uploads/dspace.jdvu.ac.in/6727-2009BT-402(1).pdf
2010 : https://www.pdfquestion.in/uploads/dspace.jdvu.ac.in/6727-2010BT%20-%20402.pdf
2011 : https://www.pdfquestion.in/uploads/dspace.jdvu.ac.in/6727-2011BT-402(3).pdf
2012 : https://www.pdfquestion.in/uploads/dspace.jdvu.ac.in/6727-2012BT-402-(4107).pdf
2013 : https://www.pdfquestion.in/uploads/dspace.jdvu.ac.in/6727-2013BT-402%20(2).pdf
WBUT Enzyme Technology Question Paper
Engineering & Management Examinations, June – 2009 :
Semester – 4 :
Time : 3 Hours
Full Marks : 70
Related : West Bengal University of Technology BT302 Biochemistry B.Tech Question Paper : www.pdfquestion.in/6725.html
GROUP – A
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the following : 10 × 1 = 10
i) Commercial streptomycin production is carried out by using
a) S.aureus b) S.griseus
c) S.pyogenes d) Streptococcus sp.
ii) Xanthan is
a) Homo polysaccharide b) Hetero polysaccharide
c) both (a) and (b) d) none of these.
iii) Frame shift mutagens intercalate into the DNA molecule and cause errors which result in
a) formation of cross-links b) formation of dimers
c) alteration of reading frames d) changes in bases.
iv) Lyophilization is the storage of commercial strain through
a) Sporulation
b) Freeze-drying
c) Boiling and subsequent condensation
d) vegetative reproduction.
v) Vitamin B 12 is produced as the by-product of
a) Streptomycin production b) Lysine production
c) Glutamic acid production d) none of these.
vi) Citric acid production is the example of
a) Bacterial fermentation b) Mold fermentation
c) both (a) and (b) d) none of these.
vii) Extremophilic enzymes are obtained more from
a) fungi b) yeast
c) virus d) archaea.
viii) Enzyme used for blood sugar measurement is
a) glucose isomerase b) glucose epimerase
c) glucose hydrogenase d) glucose oxidase.
ix) Enzyme used in fruit juice processing is
a) Pectinase b) Amylase
c) Cellulase d) Protease.
x) Between thermophilic and psychrophilic enzymes which one is useful for commercial application ?
a) Only thermophilic is useful
b) Only psychrophilic is useful
c) Both (a) and (b) are useful
d) None of these.
xi) A Newtonian fluid shows
a) a non-linear response to stress
b) deformation
c) both (a) and (b)
d) none of these.
xii) The mathematical formation for the law of conservation of mass is designated as
a) energy equation b) Arrhenius equation
c) Continuity equation d) Momentum theorem.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following questions. 3 × 5 = 15
2. What is Xanthan ? How is it produced by fermentation ? 2 + 3
3. What are base analogs ? Can they be used for strain development ? State at least two examples. 2 + 1 + 2
4. What are extremophile microorganisms ? Give a broad classification. Give two of extremophile microorganisms used commercially. 1 + 2 + 2
5. How does chemical modification of enzyme give stability ? Give at least two examples indicating enzymes and modifications done. 2 + 3
6. Write notes on Navier-Stokes equation and its application. 5
GROUP – C
( Long Answer Type Questions )
Answer any three of the following questions. 3 × 15 = 45
7. Describe in detail the requisites of a fermentation process. What do you understand by submerged and solid state fermentations ? Discuss the merits and demerits of solid state fermentation. 4 + 3 + 8
8. What damage does uv inflict on DNA ? What are the mechanisms by which damage is repaired and corrected ? Discuss how mutagenes by uv is used for strain development. 2 + 7 + 6
9. What are the different characteristics of an enzyme protein to be thermostable ? How is site directed mutagenesis done to change an amino acid ? Give two examples for successful attempt to improve stability of enzymes by a single amino acid replacement. 4 + 6 + 5
10. What are broad spectrum and narrow spectrum antibiotics ? Draw a schematic diagram for penicillin production. How is penicillin recovered ? Mention one strain involved in this production. 4 + 6 + 4 + 1
11. a) Briefly explain the experimental method for determination of flow properties of a non-Newtonian fluid such as fermentation broth.
b) A fermentation broth ( density = 1068 kg/m 3 ) is flowing through a pipe ( length 15·0 m and inside diameter 0·0528 m ) at an average velocity of 0·08 m/s. The flow properties of fluid are : consistency index = 16·0 N.S n /m 2 , flow behaviour index = 5·0 ( Dimensionless ). Calculate the pressure drop for laminar flow.