Name of the University : West Bengal University of Technology
Department : Bio-Technology
Degree : B.Tech
Sem : III
Subject Code/Name : BT-302/Microbiology
Website : wbut.ac.in
Document Type : Previous Year Examination Question Paper
Download Model/Sample Question Paper :
2009 : https://www.pdfquestion.in/uploads/ds…%20-%20302.pdf
2010 : https://www.pdfquestion.in/uploads/ds…2010BT-302.pdf
2011 : https://www.pdfquestion.in/uploads/ds…1BT-302(1).pdf
2012 : https://www.pdfquestion.in/uploads/ds…2BT-302(2).pdf
WBUT Microbiology Question Paper
Time Allotted : 3 Hours
Full Marks : 70
** The figures in the margin indicate full marks.
** Candidates are required to give their answers in their own words as far as practicable.
Related : West Bengal University of Technology BT304 Industrial Stoichiometry B.Tech Question Paper : www.pdfquestion.in/6717.html
April 2013
GROUP – A : ( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten the following : 10 × 1 = 10
i) The following substances are used in Gram staining except
a) iodine b) crystal violet
c) alcohol d) congo red.
ii) Fungi differ from bacteria in that the former
a) are eukaryotic b) contain cell walls
c) can reproduce sexually d) can form spores.
iii) Which can often live in extreme environment ?
a) Archae b) Molds
c) Yeast d) Eubacteria.
iv) Bacteria that live in hot springs have DNA with a higher content of
a) A & G b) G & C
c) A & T d) C & T.
v) In swine flu, the virus is transmitted to human from
a) chicken b) duck
c) pig d) monkey.
vi) The smallest known agents of infectious disease is
a) bacteria b) viroids
c) virus d) algae.
vii) Tuberculosis producing bacteria shows staining as
a) gram positive b) gram negative
c) acid fast stain d) do not take stain.
viii) Fungi are commonly identified by
a) thickness of mycelia b) mode of reproduction
c) fruting structure d) gram staining.
ix) Palisade arrangement of cells is found in
a) coryne bacterium diphtheriae
b) bacillus subtilis
c) saprospira sp
d) none of these.
x) The spore produced during sexual reproduction of some fungi is
a) sporangiospore b) blastospore
c) arthrospore d) ascospore.
xi) A bacterium which can grow only in the total absence of oxygen is described as
a) facultative anaerobes b) anaerobes
c) obligate aerobe d) obligate anaerobe.
xii) Anaerobic respiration of nitrate is the example of
a) assimilatory nitrate reduction
b) dissimilatroy nitrate reduction
c) nitrogen fixation
d) none of these.
GROUP – B : ( Short Answer Type Questions )
Answer any three of the following. 3 × 5 = 15
2. What are the extremophile microorgaisms ? Why are they important in biotechnological applications ? What is the evolutionary status of Archaea ? 1 + 2 + 2
3. What is peptidoglycan ? What is teichoic acid ? Write their presence and origins in Gram positive and Gram negative bacteria. 1 + 1 + 3
4. What is 16 s r RNA sequencing ? How is it helpful in taxonomy ?
5. What is enrichment of culture ? Differentiate between psychrophiles and thermophiles with example. 2 + 3
6. Give example of homofermentative and heterofermentative lactic acid bacteria. Write short notes on phosphoketolase. 2 + 3
GROUP – C : ( Long Answer Type Questions )
Answer any three of the following. 3 × 15 = 45
7. Describe asexual and sexual process of reproduction of yeast with exception. Write down the use of common yeast S.cerevisae in different biotechnology and research ( at least five ). 5 + 10
8. Briefly discuss and illustrate with suitable diagram the process of non-symbiotic nigrogen fixation. What role does leghemoglobin play in nitrogen fixation ? What is heterocysts ? What is the function of heterocysts ? 7 + 3 + 2 + 3
9. a) State at least two categories of sulphur oxidizing bacteria.
b) State at least two categories of sulphur reducing bacteria.
c) What happen when sulphate acts as terminal electron acceptor ?
d) Why some bacteria follow Entner-Doudoroff pathway ?
e) What is mixed acid fermentation ? 2 + 2 + 4 + 3 + 4
10. Write a note on cyclic photophosphorylation. Give example of purple sulphur, purple non-sulphur, green sulphur and green non-sulphur bacteria. What is nitrification ? Give example of nitrifying bacteria. What is substrate level phosphorylation ? Give example. 4 + 4 + 2 + 2 + 2 + 1
11. Explain the glyoxylate cycle in detail with a specific example of bacteria that follows it. 15
April 2012
Time Allotted : 3 Hours
Full Marks : 70
** The figures in the margin indicate full marks.
** Candidates are required to give their answers in their own words as far as practicable.
GROUP – A : ( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the following : 10 1 = 10
i) Which of the following organisms belongs to archaebacteria ?
a) Nitrosomonas b) Streptococcus
c) Mycoplasma d) Thermoplasma.
ii) Which is sequenced during the study of phylogenetic classification ?
a) mRNA b) rRNA
c) tRNA d) DNA.
iii) A family tree constructed during phylogenetic classification is
a) Dendogram b) Histogram
c) Hologram d) Cladogram.
iv) The bacterial species Nitrobacter converts
a) ammonia to nitrite
b) nitrite to nitrate
c) nitrate to atmospheric nitrogen
d) atmospheric nitrogen to amino acids.
v) Which of the following organisms belongs to hyperthermophiles ?
a) Bacillus subtilis
b) Pyrobaculum
c) Thermococcus
d) Pseudomonas.
vi) The metal ion required for enzymatic activities of nitrogenase and nitrate reductase is
a) molybdenum b) iron
c) copper d) zinc.
vii) A bacterium which can grow only in the total absence of oxygen is described as
a) facultative anaerobe
b) anaerobe
c) obligate aerobe
d) obligate anaerobe.
viii) In which of the following group of micro-organisms does the phospho ketolase pathway operate ?
a) E. coli
b) Lactobacillus species
c) Saccharomyces cerevisiae
d) Acetobacter.
ix) MacConkey’s agar is a media of which type ?
a) Selective b) Differential
c) Enrichment d) Both (a) and (b).
x) Cryopreservation of bacterial cultures is done at which temperature ?
a) – 70°C b) 0°C
c) 4°C d) – 196°C.
xi) Which of the following viruses carries out reverse transcription ?
a) Human Immunodeficiency Virus
b) Herpes virus
c) Influenza virus
d) Bacteriophage lambda
e) All of these.
xii) A plasmid
a) has a replication origin different from that of the chromosome
b) can have a higher copy number than the chromosome
c) can be separated from chromosomal DNA by a chemical procedure
d) usually carries genes that are needed only under some conditions, not genes essential under all conditions
e) all are correct.
GROUP – B : ( Short Answer Type Questions )
Answer any three of the following : 3 5 = 15
2. Why is the bacterial spore heat resistant ?
3. A bacterium grows with a generation time of 2 hours. If you inoculate 1000 cells/ml at noon on Monday, when would you expect to the culture to reach, 1,000,000 cells/ml ?
4. Identify 3 structural differences between Archaea (A) and Bacteria (B).
5. A suspension of non-growing ( stationary phase ) E. coli cells is treated with pencillin, then placed in distilled water. What will happen ?
6. What is the major function of each of the following ?
a) Poly-beta-hydroxybutyric acid
b) Fimbriae
c) Gas vacuoles.