Name of the University : North Maharashtra University
Degree : B.Sc
Department : Biotechnology
Year : I
Name Of The Subject : Methods In Biotechnology
Document type : Question Bank
Website : nmu.ac.in
Download Model/Sample Question Papers : https://www.pdfquestion.in/uploads/nmu.ac.in/5297.-F.%20Y.%20B.%20Sc.(Bio-Tech)%20Paper-II%20Question%20Bank.pdf
Methods In Biotechnology Paper – II :
QUESTIONS BANK
Class – F.Y.B.Sc. 2007-2008
Unit I- Biophysical Chemisrty
Q.1 A) Define/ Explain
1) pH
2) Buffer
Related : North Maharashtra University Fundamental Methods in Microbiology B.Sc Question Bank : www.pdfquestion.in/5296.html
3) Specific Gravity
4) Surface Tension
5) Sedimentation
6) Filtration
7) Stoichiometry
8) Adsorption
9) Distillation
B) Multiple Choice Questions
1) Bond angle of H-O-H Is——- a) 104.5oC b) 105.4oC C) 104oC d)105oC
2) H2O carries ———— a)Positive charge b)negative Charge c)no net charge
3) Melting point of water is—— a) 0oC b) 10oC c)100oC d)50oC
4) pH range is in between—– a) 0-14 b)1-14 c)0-7 d)1-7
5) pH of lemon juice is——– a)acidic b)basic c)neutral d)none of the above
6) Buffers has the ability toa) Change in pH b)resist change in pH c)no resist to change
7) Force of gravity tends to move particles in a liquid unidirectionally downward to
a) Specific gravity b) Density c) Viscosity d) Sedimentation
Q.2- Answer the following/short notes (4 marks)1) Specific gravity
2) Sedimentation3) Filtration4) Viscosity5) Stoichiometry6) Surface tension
Unit II- Microscopy
Q.1-A) Define/ Explain (2 marks)
1) Microscope
2) Microscopy
3) Magnification
4) Resolution
5) Numerical aperture
B) Multiple Choice Questions
1) The ratio of diameter of lenses to its focal length is referred as—
a) Magnification b) resolution c) Numerical aperture d) none of the above
2) ______ is the ability to reveal closely adjacent points as separate & distinct.
a) Magnification b) resolution c) Numerical aperture d) none of the above
3) If green light of wavelength 500nm objective with NA 1.4 is used, then the resolution
a)178X b)178nm c)178m d) None of the above
4) ______ controls the intensity of light entering in microscope.
a) Iris Diaphragm b) Mirror c) Condenser d) All of the above
5) In _______ type of microscope, the field surrounding a specimen appears
a) Compound microscope b)Phase contrast microscope c) Dark field microscope
Q.2- Answer the following/short notes (4 marks)
1) Numerical Aperture 2) Microtome & ultramicrotome
Unit III- General Staining Techniques
Q.1-A) Define/ Explain (2 marks)
1) Stain
2) Staining
3) Acidic Stain
4) Basic Stain
5) Intensifier
6) Fixative
7) Mordant
B) Multiple Choice Questions
1) Use of single stain to color the bacteria is commonly called as——-
a)Monochrome staining
b)Gram staining
c)Differential Staining
d)all of the above
2) A substance that forms an insoluble complex with stain & serves to fix the color to
bacterial cell is called———
a) Mordant
b) Intensifier
c) Fixative
d) none of the above
3) In Gram Staining, Gram’s iodine is act as——-
a) Counter stain
b) primary stain
c) Secondary stain
d) Mordant
4) A stain which on ionization gives positively charged molecules is referred as ______
a)Acidic Stain
b) Basic Stain
c)Anionic Stain
d) Basic mordant
5) The —- charged group of bacterial cell surface produces attraction between basic stain.
a) Negative
b) Positive
c) Neutral
d) All of the above
6) From the following, ——–is referred as differential staining technique.
a)Monochrome staining
b)Gram staining
c) Lactophenol cotton blue Staining
d)all of the above
7) In Gram Staining Safranin is act as———-
a) Primary stain
b) Mordant
c)Counter stain
d) decolorizer
8) In Gram Staining, Differentiation of microorganisms based on———
a) Cell wall
b) Plasma membrane
c) Capsule
d) All of the above
Q.2- Answer the following/short notes (4 marks)
1) Acid fast staining
2) Smear preparation
Q.3- Answer the following (6 marks) :
1) Principle & procedure ofa) Monochrome staining
b) Gram staining
c) Lactophenol cotton blue Staining
d) KOH
e) Double staining of vascular tissues
f) WBC staining
Unit IV Cell measurement
Q.1-A) Define/ Explain(2 marks)
1) Stage micrometer
2) Ocular micrometer
3) Growth
4) Micrometry
B) Multiple Choice Questions
1) Basic unit of bacterial measurement is —–
a) Micrometer
b)Nanometer
c) Milimeter
d) All of the above
2) Basic unit used for virus measurement is —–
a) Micrometer
b) Nanometer
c) Milimeter
d)All of the above
3) 1 meter is equal to—
a)109
b)10-9
c)106
d)10-6
4) Each division of stage micrometer equal; to———
a) 0.01mm
b)1 µm
3)0.1mm
d) None of the above
5) Breed methods ——–
A) Direct microscopic count method
b) Indirect microscopic count method
c) Turbidometric method
d)none of the above