Name of the College : Sphoorthy Engineering College
University : JNTUH
Department : AUTOMIBILE ENGINEERING
Subject Code/Name : 09A52304/GENETIC ENGINEERING
Year : December 2011
Degree : B.Tech
Year/Sem : III/I
Website : http://www.sphoorthyengg.ac.in/#/home
Document Type : Model Question Paper
Download Model/Sample Question Paper : https://www.pdfquestion.in/uploads/sphoorthyengg.com/4794-09A52304-GENETICENGINEERING.pdf
Sphoorthy Genetic Engineering Question Paper
III B.Tech I Semester Examinations,December 2011
Time: 3 hours
Max Marks: 75
Related : Sphoorthy Engineering College 09A52305 Fundamentals of Enzyme Mechanisms & Technology B.Tech Question Paper : www.pdfquestion.in/4791.html
Answer any FIVE Questions :
All Questions carry equal marks :
1. Write short notes on colE1plasmid and pBR327. [15]
2. Write short notes on:
(a) Homopolymer tailing
(b) Physiological significance of restriction & modification system. [7+8]
3. Comment on:
(a) Application of Homopolyer Tailing in cDNA cloning.
(b) Subtractive cloning. [15]
4. Explain the advantages & disadvantages of chemical degradation method. [15]
5. Explain the organization of the lac operon & other genes involved with lactose metabolism in E.coli. [15]
6. Write short notes on
(a) Problems encountered in PCR & their solutions.
(b) Application of PCR in modern molecular biology. [7+8]
7. Write about transgenic animals as bioreactors for the production of therapeutically important proteins. [15]
8. Discuss the significance of photolabile protecting groups in the manufacture of DNA chips. Give two examples of photosensitive groups. [15]
Set No. 2
1. Briey describe gene knock out & its applications. [15]
2. Mention the properties, a plasmid should possess for its use in recombinant DNA. [15]
3. Write the principle & procedure of ultrasonication. [15]
4. How is PCR useful in medical diagnosis & forensic science. [15]
5. Write about AFLP primers and adaptors. What are the advantages of AFLP. [15]
6. Explain the synthesis of cDNA using oligo G primer. [15]
7. Write about regulation of araBAD operon. [15]
8. Discuss the role of restriction enzymes in restriction mapping. Discuss the significance of double digestion in the process. [15]
Set No. 3
1. Write a short notes on:
(a) Application of linkers and adaptors in cDNA cloning.
(b) Vectors used for cDNA cloning. [15]
2. Discuss the basic principle involved in PCR technique in detail. [15]
3. Define recognition sites and explain their properties. [15]
4. Define gene therapy. Explain in detail ex- vivo gene therapy and its application. [15]
5. Discuss in detail the prokaryotic promoters. [15]
6. Write short notes on:
(a) Nucleotide analogs.
(b) Sequencing gel. [15]
7. Discuss the variants of RAPD in detail. [15]
8. Explain the mechanism of transposition and excision. [15]
III B.Tech I Semester Examinations,December 2011 :
Genetic Engineering : Bio-Technology
Time : 3 hours
Max Marks : 75
Answer any FIVE Questions. All Questions carry equal marks
1. Write about complementations or suppression of mertant phenotype in the cloning cell or selection of recombinant-decient phages. [15]
2. What are DNA micro chips? Write about support media & the strategies adopted for cross linking & immobilization of DNA on the surface of support media. [15]
3. Enumerate the factors that aect the PCR reaction at various steps. [15]
4. Explain the principle & steps involved in gel retardation assay. [15]
5. Write about
(a) T7 DNA polymerase and its applications
(b) Polynucleotide kinase and its applications. [15]
6. Classify plasmids based on size, copy number and function in detail. [15]
7. Discuss in detail gene delivery by non-viral systems. [15]
8. Discuss control of transcription termination in prokaryotes. [15]
Transport Phenomena In Bioprocesses :
R09Code No : 09A52301
SET-4
1.a) How do you measure the viscosity using coaxial cylinder rotary viscometer.
b) Discuss about the rheological properties of fermentation broth. [7+8]
2. Derive the equations for friction factor when the fluid is flowing in tubes. [15]
3. A plastic panel of area A=900 cm2 and thickness Y=0.6cm was found to conduct heat at a rate of 3.5 watts at steady state with temperatures of T0 =250C and T1=250C on the two main surfaces. What is the thermal conductivity of the plastic in cal/m sec K? [15]
4. Derive the temperature distribution in a Stirred Tank Reactor when the flow is turbulent. [15]
5.a) Discuss about boundary layer theory and film theory
b) What does the Corrsin equation describe? [8+7]
6.a) Define mass transfer coefficient and derive the equations to calculate mass transfer coefficient
b) Discuss about penetration theory. [7+8]
7.a) What are the various correlations for evaluating mass transfer coefficient?
b) Write short notes on liquid- liquid mass transfer? [7+8]
8.a) What are the factors affecting cellular oxygen demand ? Explain
b) Discuss about oxygen uptake in cell cultures. [8+7]