Name of the College : Sphoorthy Engineering College
University : JNTUH
Department : AUTOMIBILE ENGINEERING
Subject Code/Name : 09A52305/FUNDAMENTALS OF ENZYME MECHANISMS & TECHNOLOGY
Year : December 2011
Degree : B.Tech
Year/Sem : III/I
Website : sphoorthyengg.com
Document Type : Model Question Paper
Download Model/Sample Question Paper : https://www.pdfquestion.in/uploads/sphoorthyengg.com/4791-09A52305%20-%20FUNDAMENTALS%20OF%20ENZYME%20MECHANISM%20AND%20TECHNOLOGY.pdf
Fundamentals of Enzyme Mechanisms Question :
B. Tech III Year I Semester Examinations, December-2011
(BIO-TECHNOLOGY)
Time: 3 hours
Related : Sphoorthy Engineering College 09A52301 Transport Phenomena In Bioprocesses B.Tech Question Paper : www.pdfquestion.in/4796.html
Max. Marks: 75
Answer any five questions :
All questions carry equal marks :
1. Explain the role of enzymes in food and pharmaceutical industries. [15]
2. Discuss in detail about various techniques used in purification of enzymes.[15]
3.a) What is transition state theory.
b) Explain the effect of substrate concentration on the rate of an enzyme catalyzed reaction. [5+10]
4. What are non natural amino acids? Explain various techniques used for identification of amino acids with a suitable example. [15]
5. Discuss the following
a) Briggs -Halder equation.
b) Lineweaver–burk plot. [6+9]
6. What is isozyme? Describe in detail about various types of enzyme inhibition.[15]
7.a) How do you determine the number of active sites in enzyme.
b) Explain in detail about burst kinetics. [6+9]
8. What is meant by immobilization of enzyme? Describe different methods of immobilization of enzymes. [15]
R09Code No: 09A52305 SET-2
1.a) Give one example of the reaction catalysed by each of the following classes of enzymes.
a) Oxido – reductases b) Transferasesc) Hydrolases. d) Isomerases.
b) What is free energy of activation. [10+5]
2. Describe briefly about various techniques used in assay of enzymes. [15]
3. Explain how fluid forces, chemical agents, radiation and temperature affect the enzyme activity. [15]
4.a) Give an account of acid – base behaviour of amino acids.
b) Write about the Zwitter ion form of amino acids. [10+5]
5. Discuss the following:
a) Quasi steady state hypothesis.
b) Multiple substrate kinetics. [6+9]
6. What is the effect of the inhibitors on the reaction kinetics of the enzyme. [15]
7. Write briefly on
a) Burst kinetics of Chymotrysin.
b) Significance of enzyme kinetics. [10+5]
8. What are the effects of inhibitors, temperature, PH on immobilized enzyme catalytic activity and deactivation. [15]
R09Code No: 09A52305
SET-3
1. Write short notes on the following
a) Fischer lock and key hypothesis.
b) Medical applications of enzymes. [9+6]
2. Explain the following
a) Enzyme purification by Ammonium sulphate precipitation.
b) Enzyme concentration by Ultra filtration. [9+6]
3. Describe the factors that influence the activity of enzymes. Why do all enzymes show a PH optimum. [15]
4. Explain in detail about the physical and chemical properties of amino acids. [15]
5. Comment on the following
a) Ping Pong mechanisms.
b) Multi substrate reactions. [5+10]
6. What are suicide inhibitors. How and Why a substrate and product inhibit the enzyme catalysed reaction. [15]
7.a) Write in detail about pre-steady state kinetics.
b) Describe in detail about the importance of active sites in enzymes. [9+6]
8. Discuss the effects of external mass transfer resistance of immobilized enzyme reactions. [15]
B. Tech III Year I Semester Examinations, December-2011 :
Fundamentals Of Enzyme Mechanisms & Technology : (Bio-Technology)
Time : 3 hours
Max. Marks : 75
Answer any five questions. All questions carry equal marks
1. Write short notes on the following
a) Enzyme co-factors
b) Important industrial enzymes and their sources. [6+9]
2. Explain in detail about extraction of enzymes by chemical and physical methods. [15]
3.a) What are the factors contributing to the catalytic efficiency of enzymes.
b) What properties of enzymes confer the ability to operate under mild condition and be destroyed by extreme conditions. [12+3]
4. Comment on the following
a) Stereo chemistry of amino acids.
b) Zwitter ion.
c) Identification of amino acids by TLC. [15]
5. Write notes on
a) Double – reciprocal plot.
b) Single substrate reaction. [7+8]
6.a) Differentiate between the reversible competitive inhibition and reversible non competitive inhibition.
b) Explain briefly about uncompetitive inhibition. [10+5]
7. What is burst kinetics. Discuss about the enzyme kinetics at limiting condition. [15]
8.a) What type of bioreactors do you suggest for immobilized enzyme system.
b) Write about the diffusion effects in immobilized enzymes. [7+8]