Name of the University : The Tamilnadu Dr. M.G.R. Medical University
Degree : D.M
Branch : Branch X – Clinical Haematology
Subject Code/Name : 1401/STRUCTURE AND FUNCTION OF THE HAEMOPOIETIC SYSTEM, MOLECULAR BIOLOGY AND GENETIC ASPECTS OF HAEMOPOIESIS
Paper : I
Document Type : Question Bank
Website : web.tnmgrmu.ac.in
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Structure & Function of the Haemopoietic System :
August 2008 :
Sub. Code: 1401
D.M. DEGREE EXAMINATION
(Higher Specialities)
Branch X – Clinical Haematology
Related : The Tamilnadu Dr. M.G.R. Medical University Clinical Rheumatology & Clinical Immunology DM Question Bank : www.pdfquestion.in/4488.html
(Revised Regulations)
Paper I – STRUCTURE AND FUNCTION OF THE HAEMOPOIETIC SYSTEM, MOLECULAR BIOLOGY AND GENETIC ASPECTS OF HAEMOPOIESIS
Q.P. Code: 161401
Time: Three hours
Maximum: 100 Marks
Answer ALL questions :
Draw suitable diagrams wherever necessary. :
I. Essays: 2 x 20 = 40
1. Discuss the changes in the site, morpholosy and nature of haemopoiesis from embryonic stage to adult life with suitable diagram.
2. Discuss the structure and function of different areas of a lymph mode with suitable diagram.
II. Write short notes on: 10 x 6 = 60
1. Ferrokinetics, its application in haematology.
2. Erythropoietin.
3. Restriction Fragment length polymorphism.
4. Alternative pathway of complement activation.
5. Modern cell based theory of blood coagulation.
6. Euchromatin and Heterochromatin in a cell.
7. One carbon metabolism in haematology.
8. Histone and gene action.
9. Morphological change in myeloid cells in myelodysplasia.
10. Principles of sequencing a gene with known sequence.
August 2009 :
I. Essays: 2 x 20 = 40
1. Monocyte – Macrophage system.
2. Describe the coagulation cascade.
II. Write short notes on: 10 x 6 = 60
1. Anemia of inflammation.
2. Factor XIII.
3. RH blood group system.
4. PIG – A mutations.
5. Molecular pathogenesis of APML.
6. Pathogenesis of bone disease in myeloma.
7. NK Cell.
8. The cell cycle.
9. Immunoglobulin structure.
10. Hybridoma.
August 2011 :
I. Elaborate on :
1. Describe the hematopoietic stem cell niche and its relevance to hematopoiesis as well pathogenesis of hematological diseases.
2. Describe the current understanding of the platelet vessel wall interaction and give examples of the defects in the common platelet disorders.
II. Write notes on :
1. Clonogenic DNA (cDNA)
2. Quantitative PCR
3. Common mutations in the thalassemias and their detection methods
4. bcr-abl translocation and mutations in this region.
5. JAK 2 mutation
6. Dendritic cells
7. T cell gene receptor
8. Platelet granules
9. B cell ontogeny
10. Mesenchymal stromal cells
AUGUST 2012 :
I. Elaborate on:
1. Discuss the molecular mechanisms underlying the regulation of systemic iron homeostasis with particular emphasis on the role of hepcidin.
2. Describe the various stages of B cell lymphopoiesis including commitment and response to antigenic stimuli.
II. Write notes on:
1. Transcription factors involved in lineage selection by hematopoietic cells.
2. Stem cell mobilization.
3. Genetic defects in hemochromatosis.
4. Locus control region.
5.Telomere.
6. Hematopoietic stem cell niche.
7. WT1 antigen.
8. “ALIP” in the bone marrow.
9. Bone marrow microenvironment in multiple myeloma.
10. PCR in molecular genetics.
August 2013 :
I. Elaborate on :
1. What is angiogenesis? Briefly explain its role in haematological diseases and drugs used to inhibit angiogenesis.
2. Classify immunosuppressive drugs and briefly explain their mechanism of action with special reference to Graft Versus Host Disease.
II. Write notes on :
1. Prion diseases.
2. Gene therapy.
3. JAK inhibitors.
4. Carfilzomib.
5. Comparative genome hybridization (CGH).
6. Complement system.
7. Gap PCR.
8. FLT3 inhibitors.
9. Confirmation sensitive gel electrophoresis (CSGE).
10. Role of multimer analysis in von Willebrand disease.
Theory Syllabus :
Departments involved in the training program:
i) Clinical Hematology
ii) Immuno Hematology and Transfusion Medicine
iii) Biochemistry
iv) General Pathology
v) Nuclear Medicine
vi) Radiotherapy
I. Laboratory Hematology :
A. General Laboratory Hematology :
a) Proper use and care of common laboratory instruments such as the light microscope, Centrifuge, water baths, freezers, weighing balance etc.,
b) Weighing of solids, preparation of molar and Normal solutions, preparation and use of buffers. Familiarization with the practical concepts of pH, molarity, normality, osmolality, normal values and reference ranges.
c) The nature and uses of distilled and deionized water.
d) Blood collection of samples venupuncture and finger prick methods of sample collection, types of anticoagulants, containers and the effects of delay in processing and storage.
e) Determination of blood counts (Hemoglobin, hematocrit, total WBC and platelets) manually and calculation of red cell indices.
i) Use of automated electronic blood cell counters including principles and practice.
ii) Interpretation of peripheral blood counts.
iii) Preparation of blood films – manual and automated techniques.
iv) Staining of peripheral blood films with Romanowsky and other dyes by manual and automated techniques.
v) Review of normal and abnormal blood films with emphasis on
a) Morphology of red cells, White cells and Platelets.
b) Performance of WBC differential count.
c) Subjective assessment of platelet count.
d) Diagnostic interpretation of abnormal films.
vi) Preparation of smears of bone marrow aspirates and biopsy imprints (touch preparations).
vii) Preparation and staining of thin and thick blood films for malaria parasites.
viii) Supravital staining of reticulocytes; manual and automated counting of reticulocytes.
ix) Performance of bone marrow aspiration and trephine needle biopsy.
xi) Staining (Romanowsky dyes and Prussian Blue for iron) and diagnostic valuation of smears of bone marrow aspirate.
xii) Performance and interpretation of HbS (sickle hemoglobin) solubility test, screening for red cell G6PD activity and its interpretation.
xiii) Neutrophil function assays.