Name of the University : The Tamilnadu Dr. M.G.R. Medical University
Degree : M.Pharm DEGREE EXAMINATION
BRANCH VI : PHARMACEUTICAL BIOTECHNOLOGY
Subject Code/Name : 2918/Bioprocess Technology
Year : I
Paper : III
Document Type : Question Bank
Website : web.tnmgrmu.ac.in
Download Model/Sample Question Paper :
2011-2014 : https://www.pdfquestion.in/uploads/web.tnmgrmu.ac.in/PHARMACY/3875-262918KY.pdf
Bioprocess Technology Question :
MAY 2011 :
[KY 357] Sub. Code: 2918
M.PHARM. DEGREE EXAMINATION
(Regulations 2010)
Related / Similar Question Paper :
TNMGRMU Pharmaceutical & Cosmetic Analysis M.Pharm Question BankTNMGRMU Pharmaceutical Organic Chemistry B.Pharm Previous Year Question Paper
(Candidates admitted from 2010-2011 onwards)
BRANCH VI : PHARMACEUTICAL BIOTECHNOLOGY
PAPER III : BIOPROCESS TECHNOLOGY
Q.P. Code : 262918
Time : Three hours
Maximum : 100 marks
Answer All questions :
I. Essay Questions : (6 x 10 = 60)
1. Explain the downstream processing of extra cellular product.
2. Discuss in detail about the basic principles of fermentation.
3. Explain the primary and secondary screening techniques.
4. Describe in detail the fermentative production and recovery of Alcohol.
5. Explain the thermal death kinetics.
6. Explain the computer control of fermentation process: system configuration and application.
II. Write Short Notes : (8 x 5 = 40)
1. Measurement of mass transfer coefficient.
2. Bio- autography.
3. HTST – Sterilization.
4. Maintenance of stock cultures.
5. Fermentative production of Penicillin.
6. Microbial transformation of steroids.
7. Explain the operation of air- lift bio-rectors.
8. Metabolic response assay.
October 2011 :
Answer ALL questions in the same order. :
I. Elaborate on :
1. Explain mass transfer theory and oxygen transfer system.
2. (a) Michaelis Menton constant
(b) Preparation of fine chemical by using immobilized system.
II. Write notes on :
1. Outline about closed fermentation system
2. Line weave – burke plot
3. Application of bioreactor
4. Importance of aeration and agitation
5. Measurement of mass transfer coefficient
6. Outline about cleaning and sterilization of air
7. Briefly about biomass
8. Write brief discussion about Anti foaming agent with examples
9. Maintenance of stock culture
10. Design and types of impeller
MAY 2012 :
I. Elaborate on:
1. (a) Design and operation of large scale fermentor and its application.
(b) HTST Sterilization-Advantage and disadvantage.
2. Define biotransformation and explain biotransformation of steroids and alkaloids.
II. Write notes on:
1. Single cell protein
2. Thermal death kinetics
3. Theory of chromatography separation
4. Differentiate downstream and upstream processing
5. Equipment and design of solvent extraction
6. Computer application in bioprocessing
7. Explain cyclic AMP-Fermentation process
8. Metabolic response assay
9. Packed bed fermentor
10. Primary and secondary screening
NOVEMBER 2012 :
I. Elaborate on :
1. Explain the various techniques used in scale up of fermentation.
2. Describe in detail the fermentation production of (i) Streptomycin (ii) Vitamin B 12.
II. Write notes on :
1. Rheological properties of fermentation system.
2. Role of computer in controlling fermentation process.
3. Continuous culture method.
4. Design of a Bioreactor.
5. Principles of downstream process.
6. Factors affecting mass transfer coefficient.
7. Strain improvement techniques.
8. Regulations governing the manufacture of biological products.
9. Immobilization and its applications.
10. Microbial production of alcohol.
April 2013 :
I. Elaborate on : (2×20=40)
1. Explain about different type of cultivation system with graphical representation
2. Write detail about theory, design , operation & recovery of following downstream process
a. Solvent extraction
b. Chromatographic separation
II. Write notes on : (10×6=60)
1. Maintenance of stock cultures
2. Brief notes on Supply of air
3. Briefly about D value & Z value
4. Production and purification of penicillin
5. outline about liquid sterilization techniques
6. Discuss about different type of immobilization method
7. Steroid bio transformation
8. Design &operation hollow fibre bioreactor
9. Briefly about different type of impeller
10. Theory of fermentation process
October 2013 :
I. Elaborate on : (2 x 20 = 40)
1. Explain the various systems of cultivation and their merits and demerits.
2. Discuss in detail about the design of bio reactor and uses of Computers in fermentation.
II. Write notes on : (10 x 6 = 60)
1. Cleaning & sterilization of air.
2. Extraction methods .
3. Fermentation kinetics.
4. Air lift fermentor .
5. Preservation of microbial culture.
6. Bioprocessing of alcohol .
7. Microbial transformation.
8. Enzyme assays.
9. Importance of rheology.
10. Bio mass estimation.
April 2014 :
I. Elaborate on : (2×20=40)
1. Explain the following microbial metabolites.
a) Vitamin B12 and Riboflavin.
b) Glutamic acid and Lysine.
2. Detail discussion about rheological properties of fermentation systems and its importance.
II. Write notes on : (10×6=60)
1. Merit and demerit of HTST Sterilization
2. Application of bioreactor
3. Importance of aeration & agitation
4. Design & operation of bubble column fermentor
5. Outline about closed fermentation system
6. Advantage of bio transformation
7. Theory of downstream process
8. Brief notes on cleaning and sterilization of air
9. Application of air lift fermentor
10. Importance of pH & temperature control knob in fermentor
October 2014 :
I. Elaborate on : (2×20=40)
1. Write detail about biosynthesis of secondary metabolites and its importance.
2. Define bioreactor and explain different types of bioreactor.
II. Write notes on : (10×6=60)
1. Discuss about strain improvement for increased yield.
2. Alkaloid biotransformation.
3. Briefly about different type of sparger.
4. Production of lactic acid.
5. Measurement of mass transfer coefficient.
6. Application of semi open system.
7. Briefly about air compressing system.
8. Define microbial transformation and explain with example.
9. Explain the methods of cell disruption.
10.Compare the efficacy of immobilized and free cells.