Name of the University : The Tamilnadu Dr. M.G.R. Medical University
Degree : M.Pharm DEGREE EXAMINATION
BRANCH IV : PHARMACOGNOSY
Subject Code/Name : 2912/Biological Standardisation And Pharmacological Screening Methods
Year : I
Paper : III
Document Type : Question Bank
Website : web.tnmgrmu.ac.in
Download Model/Sample Question Paper :
2011-2014 : https://www.pdfquestion.in/uploads/web.tnmgrmu.ac.in/PHARMACY/3867-1-262912KY.pdf
1991-2000 : https://www.pdfquestion.in/uploads/web.tnmgrmu.ac.in/PHARMACY/3867-2-262862KC.pdf
2001-2011 : https://www.pdfquestion.in/uploads/web.tnmgrmu.ac.in/PHARMACY/3867-3-262862KX.pdf
Biological Standardisation & Pharmacological Screening Method :
MAY 2011 :
[KY 351] Sub. Code: 2912
M.PHARM. DEGREE EXAMINATION :
(Regulations 2010)
Related : TNMGRMU Biogenesis And Chemistry Of Natural Products M.Pharm Question Bank : www.pdfquestion.in/3865.html
(Candidates admitted from 2010-2011 onwards)
PAPER III : BIOLOGICAL STANDARDISATION AND PHARMACOLOGICAL SCREENING METHODS
Q.P. Code : 262912
Time : Three hours
Maximum : 100 marks
Answer All questions :
I. Essay Questions : (6 x 10 = 60)
1. Discuss about CPCSEA guidelines for the usage of animals for experimental purpose.
2. List out screening methods for gastric ulcer. Explain in detail about pyrolic ligation induced ulcer and NSAID’s induced ulcer.
3. What is Diabetes? Screening models for Diabetes. Discuss any one method in detail.
4. Detail the principle of Immunoassay and explain about the separation techniques.
5. Discuss about the screening methods available for anti inflammatory drugs. Explain in detail about the Freunds’s Complete Adjuvant induced anthritis.
6. What is Parkinsonism? List out the screening models for Parkinsonism and explain MPTP model in detail.
II. Write Short Notes : (8 x 5 = 40)
1. Note on Transgenic animals.
2. Discuss the different methods of blood collection.
3. Write note on invitro anti cancer activity.
4. Enumerate the different screening models anti-fertility agents and describe any one.
5. Different models in diarrhoea and laxatives. Explain any one model from diarrhoea.
6. Note on Euthanasia.
7. Define Toxicity? Explain about acute, subacute and chronic toxicity studies.
8. Paird -t- test, wilcoxon test and student t- test.
October 2011 :
(180 Min)
Answer ALL questions in the same order. :
I. Elaborate on :
1. Explain the OECD guidelines for the conduct of acute, sub-acute and chronic toxicity studies.
2. Discuss the various methods employed in the screening of antiarrhythmic drugs.
II. Write notes on :
1. Optimization of immunoassays.
2. Use of knock-out mouse in the preclinical evaluation of drugs.
3. Explain any one method for the bioassay of digitalis.
4. Conditioned avoidance test.
5. Explain any two methods for the screening of drugs used for Parkinson’s disease.
6. Student’s t test.
7. Carcinogenecity testing.
8. Randall – Selitto test.
9. Write a note on different human cell lines used in screening techniques.
10. Charcoal meal test.
MAY 2012 :
(180 Min)
Answer ALL questions in the same order. :
I. Elaborate on:
1. a. Describe the bioassay of d-tubocurarine.
b. Describe the invitro anticancer drug screening.
2. a. Explain any two invivo screening methods for antihypertensives.
b. Explain any two invivo screening methods for anticonvulsants.
II. Write notes on:
1. Explain Phrenic nerve diaphragm of rat.
2. Write short notes on principles of bioassay.
3. Describe the Shay rat model.
4. Acute toxicity studies as per OECD guidelines.
5. What are the advantages and disadvantages of alternative experimental models?
6. Describe different animal cell lines used in screening.
7. Despair swim test.
8. Euthanasia.
9. Short notes on Wilcoxon test, Paired T test.
10. Explain one chronic and one acute screening model for anti-inflammatory drugs.
NOVEMBER 2012 :
(180 Min)
Answer ALL questions in the same order. :
I. Elaborate on :
1. Explain the various models used in antihypertensive screening.
2. Discuss in detail the different methods employed in the euthanasia of laboratory animals.
II.Write Notes on :
1. MES – induced seizures.
2. Explain the bioassay of oxytocin by measurement of milk ejection pressure in a lactating rat.
3. ANOVA.
4. Maintenance of cell lines.
5. Explain the role of transgenic animal models in preclinical research.
6. Write a brief note on heterogenous immunoassays.
7. Use of Shay rats.
8. Rabbit head drop method.
9. Explain any three in vitro methods for the screening of free radical scavenging activity.
10. Principles of toxicokinetics.
April 2013 :
I. Elaborate on : (2×20=40)
1. Explain in detail the different in vivo models employed in the screening of sympathomimetic drugs.
2. a. Describe the bioassay of Digitalis using pigeon as the experimental animal.
b. Explain any two in vitro methods for the screening of hepatoprotective activity
II. Write notes on : (10×6=60)
1. Rabbit head drop method.
2. Explain any one in vitro method for the screening of immunosuppressants.
3. Different methods of bioassay.
4. Sub-acute toxicity studies according to OECD guidelines
5. Explain the general principles of immunoassays.
6. Maintenance of cell lines.
7. Mutagenecity testing.
8. Maintenance of laboratory animals.
9. Advantages of Nude mice in experimental pharmacology.
10. Spinal cat.
October 2013 :
I. Elaborate on : (2 x 20 = 40)
1. a) Enumerate the screening methods of anti-diarrhoeal drugs.
b) Enumerate anesthesia and euthanasia of commonly used experimental animals.
2. a) What is catatonia? Enumerate the screening methods for anti parkinsonism drugs.
b) What are the guidelines of CPCSEA (committee for the purpose and supervision on experimental animals) to maintain the animal house.
II. Write notes on : (10 x 6 = 60)
1. Carragenin induced paw edema
2. Eddys hot plate
3. Elevated plus maze,
4. What are the protocol preparations in immunoassay methods evaluation.
5. What are the techniques for blood collection in the laboratory animals
6. Bioassay of d-tubocurarine
7. Enumerate the screening methods for anti diabetic drugs
8. Enumerate the screening methods for free radical scavenging activity
9. Enumerate the screening methods for anti arrythmics drugs
10. Range finding tests.